| Expression pattern: |
UP |
| Associated gene: |
HMGA2, Ago2 |
| Associated microRNA: |
miR-151-3p |
| Biological function: |
CircNUP98 promotes high glucose-induced proliferation, fibrosis, inflammatory response and oxidative stress in HMCs; its knockdown alleviates these injuries and increases apoptosis. |
| Molecular mechanism: |
circNUP98 acts as a ceRNA by sponging miR-151-3p, thereby positively regulating HMGA2; circNUP98 is also packaged into serum exosomes in DN. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); fibrosis (promotes); inflammation (promotes); oxidative stress (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; ROC Analysis; Clinical Sample Validation |
| Clinical significance: |
Exosomal circNUP98 shows diagnostic value for DN patients (AUC 0.9146). |
| Description: |
circNUP98 is up-regulated in DN patient serum and in high glucose-stimulated HMCs. It mainly localizes to the cytoplasm and promotes HG-induced mesangial cell dysfunction (proliferation, fibrosis/ECM markers, inflammation, oxidative stress) by sponging miR-151-3p to upregulate HMGA2; it is also present in serum exosomes and shows diagnostic potential for DN. |
| Confidence score: |
0.8455 |