| Expression pattern: |
UP |
| Associated gene: |
LIF |
| Associated microRNA: |
miR-1299 |
| Biological function: |
promotes proliferation, migration, and invasion of OSCC cells in vitro and promotes tumor growth in vivo |
| Molecular mechanism: |
circGOLPH3 acts as a miRNA sponge for miR-1299, thereby regulating LIF expression via the circGOLPH3/miR-1299/LIF axis |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circGOLPH3 is a potential novel diagnostic or therapeutic biomarker for OSCC |
| Description: |
circGOLPH3 is up-regulated in OSCC cell lines and mainly localized in the cytoplasm. It promotes OSCC cell proliferation, migration, invasion, and xenograft tumor growth by acting as a ceRNA that sponges miR-1299, thereby increasing LIF expression through the circGOLPH3/miR-1299/LIF axis. |
| Confidence score: |
0.7753 |