| Expression pattern: |
UP |
| Associated gene: |
PD-L1 |
| Associated microRNA: |
let-7 miRNA |
| Biological function: |
Promotes NSCLC cell growth/proliferation, mobility (invasion/migration), EMT, stemness and cisplatin resistance; facilitates immune evasion by inactivating CD8+ T cells (via PD-L1 including exosomal PD-L1). |
| Molecular mechanism: |
Acts as a ceRNA (RNA sponge) for let-7 miRNA, thereby increasing PD-L1 (including exosomal PD-L1) to promote malignant phenotypes and immune evasion. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); stemness (promotes); drug resistance (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis; ELISA |
| Clinical significance: |
High circ-CPA4 expression is associated with unfavorable prognosis; higher circ-CPA4 in advanced TNM stage (III/IV) and lymphatic metastasis. |
| Description: |
circ-CPA4 is upregulated in NSCLC tissues and cell lines and acts as a ceRNA sponge for let-7 miRNA, leading to increased PD-L1 (including exosomal PD-L1). This axis promotes proliferation, invasion/EMT, stemness and cisplatin resistance, and contributes to immune evasion by suppressing CD8+ T-cell activity. |
| Confidence score: |
0.8006 |