circRNA basic information
circBase ID: hsa_circ_0000977
Name: hsa_circ_NOL10
Synonym: hsa_circRNA_0000977 / circ_0977
Host Gene: NOL10
Genomic location(hg19): chr2:10784445-10808849:-
Genomic location(hg38): chr2:10644319-10668723:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: Esophageal squamous cell carcinoma / ESCC
Disease DO ID:
3748
Disease MeSH ID:
C562729; D000077277
Disease NCIt ID:
C4024
Disease ICD11 ID:
1417891145
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma

Cell lines:

ECA109; TE12

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: TP53INP2, OSTM1, KMT2D
Associated microRNA: miR‐874‐3p, miR‐487‐5p
Biological function: promotes cell proliferation and migration; inhibits apoptosis in ESCC cells
Molecular mechanism: acts as a miRNA sponge (ceRNA) binding miR-874-3p (also reported binding miR-487-5p); potentially modulates downstream targets including TP53INP2 and KMT2D
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (promotes); Hippo/YAP (other)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay

Clinical significance:

hsa_circ_0000977 is higher in ESCC plasma and shows diagnostic value (AUC 0.9888); its expression is associated with pT stage and number of positive lymph nodes and may be used for outcome prediction

Description:

In ESCC, hsa_circ_0000977 is up-regulated in patient plasma and shows strong diagnostic performance by ROC analysis. Functionally, it promotes ESCC cell proliferation and migration and inhibits apoptosis, at least partly by acting as a miRNA sponge (binding miR-874-3p) and potentially affecting downstream targets such as TP53INP2 and KMT2D.

Confidence score:

0.7369

Other information
Title:

Upregulation of hsa_circ_0000977 participates in esophageal squamous cancer progression by sponging miR-874-3p.

Journal: Journal of clinical laboratory analysis
Published: 2022
PubMed ID: 35476874
Study type:

combined biological and clinical study

Data availability: The datasets analyzed during the current study are available from the corresponding author upon reasonable request.
Code availability: -