| Expression pattern: |
UP |
| Associated gene: |
PUM1, ETS1, PTBP3, F. nucleatum |
| Associated microRNA: |
miR-760 |
| Biological function: |
Enhances CRC cell proliferation, colony formation and tumor growth; knockdown suppresses proliferation and colony formation, induces apoptosis and causes G2/M cell cycle arrest; mediates F. nucleatum-driven CRC progression. |
| Molecular mechanism: |
F. nucleatum activates ETS1 to upregulate circPTBP3; circPTBP3 acts as a miR-760 sponge, derepressing PUM1 and promoting CRC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circPTBP3 expression positively correlates with F. nucleatum abundance, PUM1 expression, larger tumor sizes and advanced TNM stages, and negatively correlates with miR-760 levels; it may serve as a potential biomarker and therapeutic target in F. nucleatum-associated colorectal carcinogenesis. |
| Description: |
circPTBP3/hsa_circ_0003458 is a human PTBP3-derived circRNA upregulated in F. nucleatum-associated colorectal cancer. It promotes CRC proliferation, colony formation and xenograft tumor growth by sponging miR-760 and increasing PUM1 expression, while its induction is mediated by the transcription factor ETS1. Clinically, higher circPTBP3 expression correlates with F. nucleatum abundance, larger tumor size and advanced TNM stage, supporting its potential as a biomarker and therapeutic target. |
| Confidence score: |
0.8415 |