| Expression pattern: |
UP |
| Associated gene: |
FOXK2-142aa, LDHA, Fis1, AGO2 |
| Associated microRNA: |
miR-484 |
| Biological function: |
Promotes HCC progression (proliferation, invasion, metastasis) and enhances the Warburg effect; promotes mitochondrial fission. |
| Molecular mechanism: |
CircFOXK2 encodes FOXK2-142aa which interacts with LDHA to enhance LDHA Tyr10 phosphorylation; and acts as a ceRNA sponge for miR-484 to upregulate Fis1 and induce mitochondrial fission, activating the Warburg effect. |
| Biological pathway or process: |
glycolysis (promotes); mitochondrial function (other); ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Co-IP; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; Bioinformatics Analysis |
| Clinical significance: |
High circFOXK2 expression was associated with poorer overall survival (OS) and circFOXK2 was an independent prognostic factor after radical hepatectomy. |
| Description: |
CircFOXK2 (hsa_circ_0000817) is upregulated in HCC and promotes proliferation, invasion and in vivo tumor growth/metastasis. It enhances the Warburg effect by encoding FOXK2-142aa to increase LDHA Tyr10 phosphorylation and by sponging miR-484 to upregulate Fis1-driven mitochondrial fission, and high expression predicts poor OS after hepatectomy. |
| Confidence score: |
0.8904 |