| Expression pattern: |
UP |
| Associated gene: |
SP1, HIF1alpha, AluSq, AluSz6 |
| Associated microRNA: |
miR-31-5p |
| Biological function: |
promotes proliferation; promotes migration; promotes invasion; promotes metastasis; promotes EMT; induces filopodia formation |
| Molecular mechanism: |
ceRNA sponging of miR-31-5p to regulate SP1; SP1-AluSq-dependent feedback loop suppresses circ-0001875 circularization; hypoxia-induced HIF1alpha-SP1 interaction disrupts SP1-AluSq binding |
| Biological pathway or process: |
proliferation (promotes); metastasis (promotes); migration (promotes); invasion (promotes); EMT (promotes); TGF-beta/SMAD (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; divergent primers PCR; Sanger Sequencing; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); Co-IP; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
Higher circ-0001875 expression was associated with poorer overall survival in NSCLC patients; circ-0001875/miR-31-5p/SP1 axis was associated with clinical features and prognosis. |
| Description: |
circ-0001875 is up-regulated in NSCLC and acts as an oncogenic circRNA promoting proliferation, migration/invasion, metastasis, EMT and filopodia formation. It functions as a ceRNA by sponging miR-31-5p to upregulate SP1 and activate EMT-related TGFbeta/Smad2 signaling, while SP1 feeds back to inhibit circ-0001875 biogenesis via AluSq binding; hypoxia-induced HIF1alpha-SP1 interaction relieves this suppression. |
| Confidence score: |
0.8912 |