| Expression pattern: |
DN |
| Associated gene: |
RUFY3 mRNA, RUFY3, YWHAE, BAD, BCL2, AGO2 |
| Associated microRNA: |
miR-6779-5p |
| Biological function: |
inhibits lung cancer cell viability, proliferation and migration; promotes apoptosis; suppresses tumor growth in vivo |
| Molecular mechanism: |
acts as a sponge for miR-6779-5p to inhibit RUFY3 mRNA degradation; directly binds RUFY3 mRNA to enhance stability; increases RUFY3 protein to modulate YWHAE/BAD/BCL2 apoptotic signaling |
| Biological pathway or process: |
apoptosis (promotes); proliferation (inhibits); migration (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Survival Analysis; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Co-IP; Bioinformatics Analysis |
| Clinical significance: |
circSORBS1 is significantly underexpressed in lung cancer tissues, blood and cell lines, and the low expression of circSORBS1 correlated with tumour grade and prognosis; ROC curve analysis (AUC 0.7326) suggests potential diagnostic indicator |
| Description: |
circSORBS1 is down-regulated in lung cancer tissues/plasma/cell lines and functions as a tumor suppressor. It sponges miR-6779-5p and also directly binds RUFY3 mRNA to increase its stability, raising RUFY3 protein and modulating the YWHAE/BAD/BCL2 apoptotic signaling to promote apoptosis and inhibit proliferation/migration and tumor growth. |
| Confidence score: |
0.8981 |