circRNA basic information
circBase ID: hsa_circ_0000098
Name: hsa_circ_SLC30A7
Synonym: circ_0000098
Host Gene: SLC30A7
Genomic location(hg19): chr1:101372407-101387397:+
Genomic location(hg38): chr1:100906851-100921841:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; tumor tissues

Cell lines:

HCC-LM3; BEL-7404; SK-Hep1; LO2

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: MCUR1, Ago2, P-gp, MDR1, ABCB1
Associated microRNA: miR-383
Biological function: promotes HCC cell proliferation, migration and invasion; accelerates cell cycle progression; inhibits apoptosis; promotes HCC progression in vivo; promotes doxorubicin resistance
Molecular mechanism: acts as a miRNA sponge to sequester miR-383, thereby relieving repression of MCUR1 (via MCUR1 3'-UTR) and increasing MCUR1 expression; increases ATP levels and P-gp expression contributing to doxorubicin efflux and resistance
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); apoptosis (inhibits); chemoresistance (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; divergent primers PCR; RT-qPCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Survival Analysis; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)

Clinical significance:

circ_0000098 functioned as a potential diagnostic tumor marker in HCC (AUC = 0.829); high circ_0000098 expression associated with shorter survival

Description:

circ_0000098 is up-regulated in HCC and acts as an oncogenic circRNA. It mainly localizes in the cytoplasm and promotes HCC progression and doxorubicin resistance by sponging miR-383 to increase MCUR1, leading to higher ATP levels and P-gp-mediated drug efflux. Clinically, higher circ_0000098 associates with worse survival and shows diagnostic potential (ROC AUC 0.829).

Confidence score:

0.8824

Other information
Title:

Hsa_circ_0000098 is a novel therapeutic target that promotes hepatocellular carcinoma development and resistance to doxorubicin.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2022
PubMed ID: 36071480
Study type:

combined biological and clinical study

Data availability: GSE155949; GSE101850
Code availability: -