circRNA basic information
circBase ID: hsa_circ_0075804
Name: hsa_circ_E2F3
Synonym: circ-E2F3
Host Gene: E2F3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008380
MONDO name: retinoblastoma
Disease details: retinoblastoma
Disease DO ID:
 768
Disease MeSH ID:
 D012175
Disease NCIt ID:
 C7541
Disease ICD11 ID:
 1855353671
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RB tissues; normal retina tissues; mice xenograft tumor tissues
Cell lines:

ARPE-19; Y79; SO-RB50; WERI-RB-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK1
Associated microRNA: miR-204-5p
Biological function: Circ-E2F3 promoted RB cell proliferation, migration, invasion, metastasis and tumor growth, and inhibited apoptosis.
Molecular mechanism: Circ-E2F3 acted as a ceRNA by sponging miR-204-5p to positively regulate ROCK1 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

Circ-E2F3/miR-204-5p/ROCK1 axis is expected to provide a promising therapeutic target for RB.
Description:

Circ-E2F3 is upregulated in retinoblastoma tissues and cells and functions as a tumor-promoting circRNA. It promotes proliferation, migration, invasion, metastasis and xenograft tumor growth while inhibiting apoptosis, mainly through sponging miR-204-5p to increase ROCK1 expression.
Confidence score:

0.7662
Other information
Title:

Circ-E2F3 acts as a ceRNA for miR-204-5p to promote proliferation, metastasis and apoptosis inhibition in retinoblastoma by regulating ROCK1 expression.

Journal: Experimental and molecular pathology
Published: 2021
PubMed ID: 33844975
Study type:

combined biological and clinical study
Data availability: Supplementary data to this article can be found online at https://doi. org/10.1016/j.yexmp.2021.104637.
Code availability: -