| Expression pattern: |
UP |
| Associated gene: |
KCNJ9 |
| Associated microRNA: |
miR-346 |
| Biological function: |
Promotes bladder cancer cell migration, invasion, proliferation, colony formation, malignant progression, and tumor growth in vivo. |
| Molecular mechanism: |
circ-GLG1 acts as a ceRNA sponge for miR-346, relieving miR-346-mediated repression of KCNJ9 and activating TGF-beta, PI3K-AKT, and MAPK pathways via KCNJ9. |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); PI3K/AKT (promotes); MAPK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Transwell Assay; RNA-seq; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Cohort Study; Bioinformatics Analysis |
| Clinical significance: |
High circ-GLG1 expression is associated with advanced pTNM stage and poor prognosis, suggesting potential as a prognostic biomarker for bladder cancer progression. |
| Description: |
circ-GLG1 is an up-regulated GLG1-derived circRNA in bladder cancer and is associated with advanced pTNM stage and poor overall survival. Functionally, it promotes malignant phenotypes and xenograft tumor growth. Mechanistically, circ-GLG1 sponges miR-346 to derepress KCNJ9, leading to activation of TGF-beta/SMAD, PI3K/AKT, and MAPK signaling pathways. |
| Confidence score: |
0.8618 |