circRNA basic information
circBase ID: hsa_circ_0093887
Name: hsa_circ_SIRT1
Synonym: -
Host Gene: SIRT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005311
MONDO name: atherosclerosis
Disease details: atherosclerosis
Disease DO ID:
 1936
Disease MeSH ID:
 D050197
Disease NCIt ID:
 C35768
Disease ICD11 ID:
 109367356
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human renal artery wall / human renal arterial neointima; plasma; rat carotid arteries
Cell lines:

VSMCs; HASMCs; HEK293A; HUVECs
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
DN
Associated gene: NF-kappaB p65, SIRT1, Ago2
Associated microRNA: miR-132
Biological function: Inhibits inflammatory phenotypic switching of VSMCs, represses vascular inflammation, and alleviates injury-induced neointimal formation.
Molecular mechanism: circ-Sirt1 directly binds NF-kappaB p65 to sequester it in the cytoplasm and suppress NF-kappaB activity; additionally, it sponges miR-132/212 to relieve repression of SIRT1, increasing SIRT1-mediated deacetylation/inactivation of nuclear NF-kappaB p65.
Biological pathway or process:

NF-kappaB (inhibits); inflammation (inhibits); ceRNA regulation (promotes)
Detected method:
Q
H
Validation methods:

RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; ChIP / ChIP-seq; Co-IP
Clinical significance:

circ-Sirt1 is decreased in atherosclerotic tissues and CAD plasma and may act as a biomarker for detection of patients with atherosclerosis.
Description:

circ-Sirt1 is down-regulated in vascular injury/atherosclerosis-related settings and suppresses VSMC inflammatory phenotypic switching. Mechanistically, it directly binds NF-kappaB p65 to limit nuclear translocation and sponges miR-132/212 to increase SIRT1, thereby reducing p65 acetylation and NF-kappaB transcriptional activity. Its decreased level in patient tissues/plasma suggests biomarker potential in atherosclerosis/CAD.
Confidence score:

0.802
Other information
Title:

circ-Sirt1 controls NF-kappaB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells.

Journal: Nucleic acids research
Published: 2019
PubMed ID: 30820544
Study type:

combined biological and clinical study
Data availability: Supplementary Tables S1; Supplementary Tables S2; Supplementary Table S3; Supplementary Figure S1; Supplementary Figure S2; Supplementary Figure S3; Supplementary Figure S4; Supplementary Figure S5; Supplementary Figure S6; Supplementary Figure S7
Code availability: -