| Expression pattern: |
UP |
| Associated gene: |
NTRK2, PIK3CA, AKT, CDK2, CCNE1, CDK4, CDK6, CCND1 |
| Associated microRNA: |
miR-885-3p |
| Biological function: |
Promotes breast cancer cell proliferation, migration and invasion; promotes cell cycle progression; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-885-3p to regulate NTRK2 and activate PIK3CA/AKT signaling; influences cell-cycle proteins (CDK2/CCNE1, CDK4/6/CCND1). |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; Bioinformatics Analysis; Transfection; MTT; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
Higher circ_6014 expression is associated with worse histological grade (grade iii) and decreases in postoperative plasma after tumor removal; suggested as a potential biomarker/therapeutic target. |
| Description: |
hsa_circ_0006014 (circ_6014), derived from ILKAP, is up-regulated in breast cancer tissues and promotes proliferation, migration/invasion and tumor growth. It functions as a miR-885-3p sponge to upregulate NTRK2 and activate the PIK3CA/AKT pathway, influencing cell-cycle regulators (CDK2/CCNE1 and CDK4/6/CCND1). |
| Confidence score: |
0.7181 |