| Expression pattern: |
DN |
| Associated gene: |
Klotho |
| Associated microRNA: |
miR-199a-5p |
| Biological function: |
circ-ITCH suppresses gastric cancer progression by inhibiting proliferation, migration, invasion, EMT and metastasis; it also has partial effect on anticancer chemotherapy. |
| Molecular mechanism: |
circ-ITCH acts as a miR-199a-5p sponge and increases Klotho expression, thereby affecting EMT and metastasis in gastric cancer. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (other); drug resistance (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Bioinformatics Analysis; ROC Analysis |
| Clinical significance: |
circ-ITCH is related to invasion depth and may serve as a molecular biomarker for diagnosis of gastric cancer; it is proposed as a potential biomarker and target for diagnosis and therapy. |
| Description: |
circ-ITCH is down-regulated in gastric cancer tissues, cell lines and serum-derived exosomes. It functions as a tumor suppressor by sponging miR-199a-5p and increasing Klotho expression, thereby inhibiting EMT, migration, invasion, metastasis and proliferation. Clinically, low circ-ITCH is associated with invasion depth and circ-ITCH may serve as a biomarker and therapeutic target for gastric cancer. |
| Confidence score: |
0.8314 |