circRNA basic information
circBase ID: hsa_circ_0005339
Name: hsa_circ_HDAC1
Synonym: circHDAC1_004 / circ_0005339
Host Gene: HDAC1
Genomic location(hg19): chr1:32792539-32793278:+
Genomic location(hg38): chr1:32326938-32327677:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissue; paracancerous tissues / paraneoplastic tissues

Cell lines:

Huh7; MHCC97H; HepG2; Hep3B; MHCCLM3; SK-Hep1; YY8103; HHL-5; HUVECs; HEK-293T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: NACC1, Ago2
Associated microRNA: miR-361-3p
Biological function: promotes proliferation, migration, invasion, stemness, EMT, angiogenesis, and metastasis in HCC
Molecular mechanism: Exosomal circHDAC1_004 acts as a miRNA sponge for miR-361-3p to upregulate NACC1 and promote EMT and malignant phenotypes.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); EMT (promotes); stemness (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Sanger Sequencing; divergent primers PCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; IF (Immunofluorescence); Bioinformatics Analysis

Clinical significance:

High circHDAC1_004 expression is associated with shorter overall survival in HCC patients.

Description:

circHDAC1_004 is up-regulated in HCC and its high expression indicates poorer overall survival. It mainly localizes to the cytoplasm, is transported via exosomes, and promotes HCC proliferation, migration/invasion, stemness, EMT, metastasis, and angiogenesis by sponging miR-361-3p to upregulate NACC1.

Confidence score:

0.8871

Other information
Title:

Exosome-transported circHDAC1_004 Promotes Proliferation, Migration, and Angiogenesis of Hepatocellular Carcinoma by the miR-361-3p/NACC1 Axis.

Journal: Journal of clinical and translational hepatology
Published: 2023
PubMed ID: 37577235
Study type:

combined biological and clinical study

Data availability: The qPCR, western blot, and other data used to support the findings of this study are included within the article.
Code availability: -