| Expression pattern: |
UP |
| Associated gene: |
ABCC1, Ago2, MDR1, Cleaved-caspase-3 |
| Associated microRNA: |
miR-193a-5p, miR-98-5p |
| Biological function: |
circ_0048856 promotes DDP resistance, tumor growth, proliferation and invasion, and suppresses apoptosis in lung cancer cells; circ_0048856 knockdown re-sensitizes DDP-resistant lung cancer cells to DDP and suppresses tumorigenesis in vitro and in vivo. |
| Molecular mechanism: |
circ_0048856 acts as a ceRNA/miRNA sponge for miR-193a-5p and miR-98-5p to regulate ABCC1, thereby promoting DDP resistance and tumorigenesis in lung cancer. |
| Biological pathway or process: |
ceRNA regulation (promotes); drug resistance (promotes); chemoresistance (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0048856 is highly expressed in DDP-resistant lung cancer tissues and may be a therapeutic target for improving cisplatin treatment efficacy. |
| Description: |
circ_0048856 is up-regulated in DDP-resistant lung cancer tissues and cells. It promotes cisplatin resistance, proliferation, invasion and tumor growth while inhibiting apoptosis, mainly by sponging miR-193a-5p and miR-98-5p to regulate ABCC1. Knockdown of circ_0048856 restores DDP sensitivity and suppresses tumorigenesis in vitro and in a xenograft model. |
| Confidence score: |
0.8174 |