| Expression pattern: |
UP |
| Associated gene: |
HIF-2alpha, NRP1, AGO2, ERK, AKT |
| Associated microRNA: |
miR-653-5p, miR-338-3p |
| Biological function: |
promotes migration and invasion (metastasis) of gastric cancer cells under a long-term hypoxic microenvironment |
| Molecular mechanism: |
Upregulated by HIF-2alpha; acts as a miRNA sponge for miR-653-5p and miR-338-3p to upregulate NRP1, activating downstream ERK and AKT signaling |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); ERK (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; Transwell Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circHIPK3 might be a long-term hypoxic biomarker and a potential prognostic biomarker for GC patients in the future |
| Description: |
In gastric cancer under long-term hypoxia, circHIPK3 is induced by HIF-2alpha and promotes migration/invasion. It functions as a ceRNA by sponging miR-653-5p and miR-338-3p to upregulate NRP1, thereby activating ERK and AKT signaling to enhance metastatic potential. |
| Confidence score: |
0.7399 |