circRNA basic information
circBase ID: -
Name: hsa_circ_GCLC
Synonym: circular RNA GCLC / circGCLC
Host Gene: GCLC
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005298
MONDO name: osteoporosis
Disease details: Osteoporosis / OP
Disease DO ID:
 11476
Disease MeSH ID:
 D010024
Disease NCIt ID:
 C3298
Disease ICD11 ID:
 2113001430
Disease OMIM ID:
 166710
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow samples
Cell lines:

-
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: GNAS, Ago2
Associated microRNA: miR-516b-5p
Biological function: promotes osteogenic differentiation; activates MAPK signalling pathway; increases osteogenesis-related proteins (RUNX2, OSX, OCN); increases mineralization and ALP
Molecular mechanism: ceRNA/miRNA sponge mechanism: circGCLC competitively binds miR-516b-5p to mediate GNAS expression, promoting osteogenesis and MAPK activation
Biological pathway or process:

MAPK (promotes); proliferation (other); osteogenic differentiation (other); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); FISH / smFISH; Western Blot
Clinical significance:

-
Description:

circGCLC promotes osteogenic differentiation of human BMSCs by acting as a ceRNA that sponges miR-516b-5p, thereby relieving repression of GNAS. This axis enhances MAPK pathway activation (p-JNK, p-p38) and increases osteogenic markers and mineralization.
Confidence score:

0.5871
Other information
Title:

Circular RNA GCLC promotes osteogenic differentiation of bone marrow mesenchymal stem cells via modulating miR-516b-5p/GNAS axis.

Journal: Journal of orthopaedic surgery and research
Published: 2025
PubMed ID: 40781640
Study type:

biological research
Data availability: No datasets were generated or analysed during the current study.
Code availability: -