circRNA basic information
circBase ID: -
Name: hsa_circ_ARHGEF28
Synonym: circ-ARHGER28
Host Gene: ARHGEF28
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; paracarcinoma tissues; peripheral blood; plasma samples
Cell lines:

MCF-10A; MDAMB-231; MCF-7; HCC-1937
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: PI3K, AKT, mTOR, Bcl-2, PARP-1, caspase-3, caspase-8, caspase-9, cleavedcaspase 3, cleavedcaspase 8, cleavedcaspase 9, BAX/Bcl2
Associated microRNA: -
Biological function: Inhibits MCF-7 cell proliferation, colony formation and migration, induces G2/M cell-cycle arrest, and increases apoptosis.
Molecular mechanism: circ-ARHGER28 may suppress breast cancer cell proliferation and promote apoptosis through association with the PI3K/AKT/mTOR signaling pathway and predicted miRNA sponging.
Biological pathway or process:

PI3K/AKT/mTOR (inhibits); apoptosis (promotes); proliferation (inhibits); migration (inhibits); cell cycle (other); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; RNA-seq; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Wound Healing Assay; Western Blot; ROC Analysis; Bioinformatics Analysis
Clinical significance:

circ-ARHGER28 could be a potential biomarker for BC diagnosis and treatment, with reported ROC diagnostic value in BC tissues and peripheral blood.
Description:

circ-ARHGER28 is down-regulated in breast cancer tissues, peripheral blood, and breast cancer cell lines, and shows diagnostic biomarker potential. Functionally, circ-ARHGER28 overexpression suppresses MCF-7 proliferation, colony formation, and migration while inducing G2/M arrest and apoptosis, likely through regulation of apoptosis-related genes/proteins and the PI3K/AKT/mTOR signaling pathway.
Confidence score:

0.7899
Other information
Title:

Clinical Diagnostic Value of circ-ARHGER28 for Breast Cancer and its Effect on MCF 7 Cell Proliferation and Apoptosis.

Journal: Anticancer research
Published: 2024
PubMed ID: 38925846
Study type:

combined biological and clinical study
Data availability: TableS1PrimersequencesofquantitativerealtimePCR.docx; FigS1.tiff; raw data uploaded as supplementary les
Code availability: -