circRNA basic information
circBase ID: hsa_circ_0007534
Name: hsa_circ_DDX42
Synonym: -
Host Gene: DDX42
Genomic location(hg19): chr17:61869771-61877977:+
Genomic location(hg38): chr17:63792411-63800617:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

cancer tissues; adjacent normal tissues; BC tissues
Cell lines:

MCF-1; MCF-7; MDA-MB-231; MDA-MB-468; SKBR-3; MDA-MB-453; MCF-10A
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MUC19
Associated microRNA: miR-593
Biological function: hsa_circ_0007534 acts as an oncogene in breast cancer; knockdown inhibits BC cell proliferation, viability, colony formation and invasion, promotes apoptosis, and induces G1 phase accumulation with reduced S and G2/M phases.
Molecular mechanism: hsa_circ_0007534 functions as a miR-593 sponge, negatively regulates miR-593, and promotes MUC19 expression through the hsa_circ_0007534/miR-593/MUC19 regulatory loop.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes)
Detected method:
Q
Validation methods:

Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Luciferase Reporter Assay; circRIP; Western Blot
Clinical significance:

hsa_circ_0007534 is significantly upregulated in BC tissues and may have an oncogenic role and therapeutic relevance in BC.
Description:

hsa_circ_0007534 is upregulated in breast cancer tissues and cell lines and functions as an oncogenic circRNA. Its knockdown suppresses BC cell viability, proliferation, colony formation and invasion, promotes apoptosis, and alters cell-cycle distribution. Mechanistically, hsa_circ_0007534 sponges miR-593 to relieve repression of MUC19, forming a hsa_circ_0007534/miR-593/MUC19 regulatory axis that promotes BC progression.
Confidence score:

0.7595
Other information
Title:

Downregulation of hsa_circ_0007534 suppresses breast cancer cell proliferation and invasion by targeting miR-593/MUC19 signal pathway.

Journal: Biochemical and biophysical research communications
Published: 2018
PubMed ID: 30139516
Study type:

combined biological and clinical study
Data availability: Supplementary data related to this article can be found at https://doi.org/10.1016/j.bbrc.2018.08.007.
Code availability: -