| Expression pattern: |
DN |
| Associated gene: |
LGALS3BP, NF-kappaB, eIF4A3 |
| Associated microRNA: |
miR-671-5p |
| Biological function: |
Inhibits prostate cancer cell proliferation, migration, and invasion; suppresses xenograft tumor growth; promotes apoptosis rather than affecting cell cycle. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-671-5p to prevent degradation of LGALS3BP, thereby blocking activation of the NF-kappaB pathway; eIF4A3 is implicated in regulating circARHGEF28 expression. |
| Biological pathway or process: |
NF-kappaB (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circARHGEF28 is down-regulated in prostate cancer and functions as a tumor suppressor by inhibiting proliferation, migration, and invasion in vitro and reducing xenograft growth in vivo. Mechanistically, it sponges miR-671-5p to restore LGALS3BP and thereby inhibits NF-kappaB pathway activation. |
| Confidence score: |
0.8635 |