| Expression pattern: |
UP |
| Associated gene: |
NACC1 |
| Associated microRNA: |
miR-136-5p |
| Biological function: |
Circ_0027089 functioned as an oncogene in HBV-related HCC; it promoted cell proliferation, colony formation, cell cycle progression, migration, invasion and tumor growth, and inhibited apoptosis. |
| Molecular mechanism: |
Circ_0027089 acts through a ceRNA mechanism by competitively targeting miR-136-5p to upregulate NACC1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Circ_0027089 was suggested as a novel biomarker for the treatment of HBV-related HCC; previous evidence regarded it as a promising marker for diagnosis of HBV-associated HCC. |
| Description: |
Circ_0027089 is up-regulated in HBV-related HCC tissues and cell lines and acts as an oncogenic circRNA. It promotes proliferation, cell cycle progression, migration, invasion and xenograft tumor growth while suppressing apoptosis, mainly through the circ_0027089/miR-136-5p/NACC1 ceRNA axis. |
| Confidence score: |
0.7053 |