| Expression pattern: |
UP |
| Associated gene: |
NUAK2, NUAK Family Kinase 2, PCNA, MMP-2, Bax |
| Associated microRNA: |
miR-378a-3p |
| Biological function: |
Promotes BC cell proliferation, migration, invasion, angiogenesis, tumor growth and migration, and inhibits apoptosis and cell cycle arrest. |
| Molecular mechanism: |
circ_0000033 functions as a ceRNA by sequestering miR-378a-3p, thereby releasing and up-regulating NUAK2 in BC cells. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); cell cycle (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circ_0000033 may be a promising therapeutic target for BC treatment. |
| Description: |
circ_0000033 is up-regulated in breast cancer tissues and cells. It promotes breast cancer proliferation, migration, invasion, angiogenesis, metastasis and xenograft tumor growth while inhibiting apoptosis and cell cycle arrest. Mechanistically, circ_0000033 sponges miR-378a-3p to release and up-regulate NUAK2, forming a circ_0000033/miR-378a-3p/NUAK2 ceRNA network and suggesting therapeutic potential in BC. |
| Confidence score: |
0.8175 |