| Expression pattern: |
UP |
| Associated gene: |
MMP9 |
| Associated microRNA: |
miR-491-5p |
| Biological function: |
circ0001361 promotes bladder cancer cell migration, invasion and metastasis in vitro and in vivo, but has no obvious effect on cell proliferation or cell cycle progression. |
| Molecular mechanism: |
circ0001361 acts as a miR-491-5p sponge to upregulate MMP9 expression, thereby promoting bladder cancer cell invasion and metastasis. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ0001361 expression is positively correlated with pathologic grade, clinical stage, vessel invasion and muscle invasion, and is associated with poor overall survival in bladder cancer patients; it may serve as a potential diagnostic, prognostic or therapeutic target. |
| Description: |
hsa_circ_0001361/circ0001361 is an up-regulated FNDC3B-derived circRNA in bladder cancer. It mainly localizes in the cytoplasm and promotes bladder cancer migration, invasion and metastasis through a circ0001361/miR-491-5p/MMP9 ceRNA axis. High circ0001361 expression correlates with aggressive clinicopathological features and poor overall survival, suggesting potential prognostic and therapeutic value. |
| Confidence score: |
0.8769 |