| Expression pattern: |
UP |
| Associated gene: |
MACC1 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes proliferation, cell cycle progression, migration and invasion, and inhibits apoptosis in colorectal carcinoma cells; promotes tumorigenesis in vivo |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0005100 sponges miR-145-5p to relieve repression of MACC1 |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Clinical Sample Validation |
| Clinical significance: |
High circ_FMN2 expression was associated with lymph node metastasis, TNM stage, and tumor size in CRC patients. |
| Description: |
hsa_circ_0005100 is up-regulated in colorectal carcinoma and mainly localized in the cytoplasm. It promotes CRC cell proliferation, cell-cycle progression, migration and invasion while inhibiting apoptosis by sponging miR-145-5p to upregulate MACC1, and its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.7701 |