circRNA basic information
circBase ID: -
Name: hsa_circ_AGFG1
Synonym: circAGFG1
Host Gene: AGFG1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; lung tissue
Cell lines:

MG63; U2OS; SAOS2; HOS; 143B; FOB; NIH3T3; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: LATS2
Associated microRNA: miR-302a, miR-302a-3p
Biological function: promotes proliferation, migration and invasion; promotes stemness; promotes tumor growth and metastasis in vivo
Molecular mechanism: Acts as a miRNA sponge/ceRNA by binding miR-302a-3p, thereby relieving repression of LATS2.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes); Hippo/YAP (other)
Detected method:
Q
Validation methods:

RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis
Clinical significance:

-
Description:

circAGFG1 is up-regulated in osteosarcoma cell lines and promotes proliferation, invasion/migration, metastasis, and stemness. Mechanistically, it functions as a ceRNA by binding miR-302a-3p to upregulate LATS2, supporting osteosarcoma progression.
Confidence score:

0.5997
Other information
Title:

CircAGFG1 Promotes Osteosarcoma Progression and Stemness by Competing with miR-302a-3p to Upregulate the Expression of LATS2.

Journal: Evidence-based complementary and alternative medicine : eCAM
Published: 2022
PubMed ID: 35958928
Study type:

biological research
Data availability: The data used to support the findings of this study are available from the corresponding author upon request.
Code availability: -