| Expression pattern: |
DN |
| Associated gene: |
PFN1 |
| Associated microRNA: |
miR-96-5p |
| Biological function: |
circ_0000972 acts as an antitumor factor in HCC; its overexpression inhibits HCC CSC colony formation, sphere formation, stemness, EMT, migration, invasion, and xenograft tumor growth. |
| Molecular mechanism: |
circ_0000972 functions as a molecular sponge for miR-96-5p, thereby upregulating PFN1 expression and suppressing HCC CSC stemness and progression. |
| Biological pathway or process: |
stemness (inhibits); EMT (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Western Blot; Transwell Assay; In Vivo Animal Model |
| Clinical significance: |
circ_0000972 has potential as a prognostic marker and a promising avenue for targeted interventions in HCC. |
| Description: |
circ_0000972 is downregulated in HCC tissues and cells and functions as a tumor-suppressive circRNA. It suppresses HCC cancer stem cell colony formation, sphere formation, EMT, migration, invasion, and xenograft tumor growth by sponging miR-96-5p and promoting PFN1 expression. |
| Confidence score: |
0.6521 |