| Expression pattern: |
UP |
| Associated gene: |
Ago2, PRDM16, UCP1 |
| Associated microRNA: |
miR-133 |
| Biological function: |
Promotes differentiation of preadipocytes into brown-like cells, enhances WAT browning, increases adipocyte metabolic activity, oxygen consumption and heat production, and aggravates tumor cachexia. |
| Molecular mechanism: |
Exosome-delivered ciRS-133 acts as a miR-133 sponge, suppresses miR-133 function, up-regulates PRDM16 and activates UCP1 to promote WAT browning and cachexia-associated metabolic dysfunction. |
| Biological pathway or process: |
ceRNA regulation (promotes); other pathway/process (promotes); mitochondrial function (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Northern Blot; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Oil Red O Staining; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Plasma exosomal ciRS-133 is up-regulated in GC, positively linked with BAT mass, correlated with body fat rate, and may be a regulator or potential therapeutic target for cancer cachexia-related metabolic disorders. |
| Description: |
ciRS-133 is a human exosomal circRNA up-regulated in plasma exosomes and tumor tissues of GC patients. Gastric cancer cell-derived exosomal ciRS-133 is delivered into preadipocytes, where it sponges miR-133 and activates the PRDM16/UCP1 axis, promoting WAT browning, metabolic activation, and cancer cachexia. |
| Confidence score: |
0.8061 |