| Expression pattern: |
UP |
| Associated gene: |
HIF1A, DYRK1A, SFPQ, SAM68, PCBP1, OGT, ZEB2 |
| Associated microRNA: |
- |
| Biological function: |
promotes EMT and metastasis; facilitates ZEB2 translation under hypoxia |
| Molecular mechanism: |
Hypoxia/HIF1A transcriptionally increases PRELID2 pre-mRNA; DYRK1A-SFPQ-SAM68 complex promotes circPRELID2 circularization; circPRELID2 binds PCBP1 and promotes its cytoplasmic retention; circPRELID2 scaffolds OGT-PCBP1 interaction to enhance PCBP1 O-GlcNAcylation (Thr99), reducing PCBP1 binding to ZEB2 3′-UTR and relieving translational silencing of ZEB2. |
| Biological pathway or process: |
EMT (promotes); metastasis (promotes); migration (promotes); invasion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; ISH (In Situ Hybridization); Survival Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; Western Blot; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; IF (Immunofluorescence); IHC (Immunohistochemistry) |
| Clinical significance: |
High circPRELID2 is associated with lymph node invasion and vascular invasion, and predicts worse OS/DFS in GC patients. |
| Description: |
In gastric cancer, hypoxia induces circPRELID2 via HIF1A and promotes its circularization via a DYRK1A-SFPQ-SAM68/Alu-dependent mechanism. circPRELID2 acts as a protein-interacting scaffold that retains PCBP1 in the cytoplasm and enhances OGT-mediated PCBP1 O-GlcNAcylation (Thr99), relieving PCBP1-mediated translational repression of ZEB2 to promote EMT and metastasis. High circPRELID2 in tumors associates with invasion features and poorer survival. |
| Confidence score: |
0.8994 |