| Expression pattern: |
UP |
| Associated gene: |
PAK1 |
| Associated microRNA: |
miR-615-5p |
| Biological function: |
Promotes NSCLC malignant behaviors (proliferation, migration, invasion), enhances radioresistance, inhibits apoptosis, and supports tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-615-5p to upregulate PAK1; validated by luciferase reporter and Ago2-RIP, with rescue experiments using miR-615-5p inhibitor or PAK1 overexpression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); radioresistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; Clinical Sample Validation; Cohort Study; Survival Analysis; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High hsa_circ_0004396 expression is associated with poorer overall survival and correlates with TNM stage and lymph node metastasis. |
| Description: |
hsa_circ_0004396 (circHUWE1) is upregulated in NSCLC and promotes malignant phenotypes and radioresistance. Mechanistically, it sponges miR-615-5p to increase PAK1 expression, and its knockdown suppresses tumor growth in a nude-mouse xenograft model. |
| Confidence score: |
0.8426 |