circRNA basic information
circBase ID: hsa_circ_0001361
Name: hsa_circ_FNDC3B
Synonym: circFNDC3B
Host Gene: FNDC3B
Genomic location(hg19): chr3:171830241-171851336:+
Genomic location(hg38): chr3:172112451-172133546:+
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004958
MONDO name: oral cavity squamous cell carcinoma
Disease details: oral squamous cell carcinoma / OSCC
Disease DO ID:
 0050866
Disease MeSH ID:
 -
Disease NCIt ID:
 C4833
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; paracancerous tissue; serum; metastatic LNs
Cell lines:

HSC3; CAL27; HN6; SCC4; HN4; FADU; SSC9; HOK; HUVEC; HLEC; 293T
In vivo animal model:

cell line-derived xenograft; other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: FUS, MDM2, HIF1A, VEGFA, SERPINE1, PROX1, ESM1, AGO2
Associated microRNA: miR-181c-5p
Biological function: Promotes migration, invasion, EMT/p-EMT, angiogenesis, lymphangiogenesis, and lymph node metastasis in OSCC.
Molecular mechanism: Binds RBP FUS and promotes its MDM2-mediated ubiquitination/degradation, stabilizing HIF1A and increasing VEGFA transcription to enhance angiogenesis; functions as a miR-181c-5p sponge to upregulate SERPINE1 and PROX1 (and related targets) to drive p-EMT/EMT and lymphangiogenesis.
Biological pathway or process:

angiogenesis (promotes); lymphangiogenesis (promotes); metastasis (promotes); migration (promotes); invasion (promotes); EMT (promotes); VEGF/VEGFR (promotes); ceRNA regulation (promotes); ubiquitination (promotes)
Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; ROC Analysis; Survival Analysis; Bioinformatics Analysis; Transfection; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Co-IP; ChIP / ChIP-seq; EdU Staining; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence)
Clinical significance:

High hsa_circ_0001361 (circFNDC3B) expression is associated with LN metastasis and poor recurrence-free survival; serum hsa_circ_0001361 is higher in OSCC patients and shows diagnostic value by ROC analysis (AUC 0.7437).
Description:

circFNDC3B is up-regulated in OSCC and correlates with LN metastasis and poor RFS. It promotes OSCC migration/invasion and enhances angiogenesis and lymphangiogenesis via a nuclear circFNDC3B-FUS/MDM2-HIF1A-VEGFA mechanism and a cytoplasmic ceRNA mechanism sponging miR-181c-5p to increase SERPINE1 and PROX1.
Confidence score:

0.9033
Other information
Title:

circFNDC3B Accelerates Vasculature Formation and Metastasis in Oral Squamous Cell Carcinoma.

Journal: Cancer research
Published: 2023
PubMed ID: 36811957
Study type:

combined biological and clinical study
Data availability: GEO: GSE131182; GSE197138
Code availability: -