| Expression pattern: |
DN |
| Associated gene: |
DLG4, G6PD, YTHDF2, AGO2 |
| Associated microRNA: |
miR-663b |
| Biological function: |
Inhibits CRC cell proliferation, migration, invasion, tumorigenesis and metastasis; suppresses the pentose phosphate pathway. |
| Molecular mechanism: |
m6A reader YTHDF2 mediates decay of m6A-modified circ_0003215; circ_0003215 acts as a ceRNA sponging miR-663b to upregulate DLG4; DLG4 inhibits PPP by promoting K48-linked ubiquitination of G6PD. |
| Biological pathway or process: |
pentose phosphate pathway (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other); ubiquitination (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Microarray; RNA-seq; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; MeRIP / MeRIP-seq; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Co-IP |
| Clinical significance: |
Low circ_0003215 level was associated with larger tumor size, higher TNM stage, and lymph node metastasis; suggested as a promising early diagnostic biomarker. |
| Description: |
circ_0003215 is down-regulated in colorectal cancer and functions as a tumor suppressor. It is degraded in an m6A-dependent manner via the m6A reader YTHDF2, and inhibits malignancy and PPP metabolism by sponging miR-663b to increase DLG4, which promotes K48-linked ubiquitination of G6PD. |
| Confidence score: |
0.8657 |