| Expression pattern: |
UP |
| Associated gene: |
STAT2, ACC1, CPT1A |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation; promotes migration; promotes epithelial-mesenchymal transition; promotes lipid synthesis; promotes tumor growth and metastasis |
| Molecular mechanism: |
circCAPRIN1 directly binds STAT2 and recruits it to activate ACC1 transcription, leading to ACC1-dependent lipid metabolic reprogramming that promotes CRC progression. |
| Biological pathway or process: |
lipid metabolism (promotes); proliferation (promotes); migration (promotes); EMT (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; ChIP / ChIP-seq; RNA-seq; Bioinformatics Analysis; IHC (Immunohistochemistry); H&E Staining; In Vivo Animal Model; Oil Red O Staining; IF (Immunofluorescence) |
| Clinical significance: |
circCAPRIN1 expression was positively correlated with the clinical stage and unfavorable prognosis of CRC patients; high circCAPRIN1 expression had unfavorable OS and DFS. |
| Description: |
circCAPRIN1 (hsa_circ_0021639) is up-regulated in colorectal cancer and promotes proliferation, migration, EMT, tumor growth and metastasis. Mechanistically, it localizes mainly to the nucleus and binds STAT2 to enhance ACC1 transcription, driving ACC1-dependent lipid synthesis. High circCAPRIN1 expression is associated with advanced stage and worse OS/DFS. |
| Confidence score: |
0.8869 |