| Expression pattern: |
DN |
| Associated gene: |
YAF2 |
| Associated microRNA: |
miR-26b / miR-26b-5p |
| Biological function: |
circRGPD6 suppresses breast cancer stem cell-like/tumor-initiating properties, proliferation, invasion and metastasis; TV-circRGPD6 enhances DNA damage marker p-H2AX, reduces CD44+CD24- stem-like populations, and synergizes with docetaxel to eradicate BCSCs. |
| Molecular mechanism: |
circRGPD6 functions as a miRNA sponge for miR-26b/miR-26b-5p, thereby upregulating YAF2 and suppressing BCSC-mediated metastasis; TV-circRGPD6 also sensitizes BCSC-mediated metastasis to docetaxel by damaging DNA of BCSCs. |
| Biological pathway or process: |
stemness (inhibits); metastasis (inhibits); invasion (inhibits); proliferation (inhibits); apoptosis (promotes); ceRNA regulation (other); drug resistance (inhibits); chemoresistance (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
B
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Northern Blot; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; TUNEL; In Vivo Animal Model; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis; Microarray |
| Clinical significance: |
High/positive circRGPD6 expression is associated with favorable prognosis, longer disease-free survival and overall survival in breast cancer patients; circRGPD6 expression is positively associated with YAF2 expression and is lower in metastatic disease. |
| Description: |
circRGPD6 is down-regulated in breast cancer, especially metastatic lesions and BCSCs. Restoring circRGPD6 with TV-circRGPD6 suppresses BCSC stem-like properties, proliferation and metastasis, partly by sponging miR-26b/miR-26b-5p and increasing YAF2 expression, and it synergizes with docetaxel. Clinically, high circRGPD6 expression predicts better DFS and OS in breast cancer patients. |
| Confidence score: |
0.8855 |