| Expression pattern: |
DN |
| Associated gene: |
PPP2R1A, PP2A, STAT3, AKT, IL-2, IFN-gamma, TNF-alpha |
| Associated microRNA: |
- |
| Biological function: |
In RA, decreased circ-CCDC134 is linked to higher disease activity; mechanistically, circ-CCDC134 inhibits PP2A to increase STAT3/AKT phosphorylation and promote IL-2 and IFN-gamma (and TNF-alpha mRNA in Jurkat) expression/secretion, reversing chronic TNF-alpha-induced T cell dysfunction. |
| Molecular mechanism: |
circ-CCDC134 binds PPP2R1A and suppresses PP2A activity, thereby enhancing STAT3 and AKT phosphorylation and increasing IL-2/IFN-gamma expression and secretion. |
| Biological pathway or process: |
PI3K/AKT (promotes); JAK/STAT (promotes); inflammation (promotes); proliferation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; RNA Pull-Down; Transfection; RIP (RNA Immunoprecipitation); Western Blot; ELISA; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circ-CCDC134 is down-regulated in RA T cells and its expression is inversely associated with disease activity (DAS28-ESR). |
| Description: |
circ-CCDC134 is down-regulated in RA T cells and inversely correlates with disease activity. It binds PPP2R1A to inhibit PP2A, increasing AKT/STAT3 phosphorylation and promoting IL-2 and IFN-gamma production/secretion, which counteracts chronic TNF-alpha-induced T-cell functional suppression. |
| Confidence score: |
0.7689 |