| Expression pattern: |
DN |
| Associated gene: |
eIF3h, METTL3, BRD4 |
| Associated microRNA: |
miR-762 |
| Biological function: |
inhibits proliferation; inhibits colony formation; inhibits invasion; inhibits migration; suppresses tumor growth |
| Molecular mechanism: |
Exosomal circLPAR1 is internalized by CRC cells and directly binds eIF3h, suppressing the METTL3-eIF3h interaction and decreasing BRD4 translation. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); migration (inhibits); other pathway/process (inhibits); mRNA stability (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; FISH / smFISH; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; circRNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Plasma exosomal circLPAR1 distinguishes CRC patients from cancer-free controls (AUC 0.858) and improves diagnostic performance combined with CEA and CA19-9 (AUC 0.875); high circLPAR1 level is associated with better overall survival. |
| Description: |
Exosomal circLPAR1 is down-regulated in CRC tissues and plasma exosomes and can serve as a CRC-specific diagnostic biomarker. Functionally, exosomal circLPAR1 inhibits malignant phenotypes and tumor growth by binding eIF3h and disrupting METTL3-eIF3h-dependent translation of BRD4. |
| Confidence score: |
0.8844 |