| Expression pattern: |
UP |
| Associated gene: |
EZH2 |
| Associated microRNA: |
miR-26b-5p |
| Biological function: |
Promotes doxorubicin (DXR) chemoresistance and malignant phenotypes (colony formation, invasion/migration) and inhibits apoptosis in osteosarcoma; circ_ANKIB1 knockdown suppresses DXR-resistant tumor growth in vivo. |
| Molecular mechanism: |
ceRNA mechanism: circ_ANKIB1 sponges miR-26b-5p to relieve repression of EZH2, increasing downstream drug-resistance proteins (MRP1, P-gp, LRP) and DXR resistance. |
| Biological pathway or process: |
chemoresistance (promotes); apoptosis (inhibits); invasion (promotes); migration (promotes); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circ_ANKIB1 expression is positively correlated with TNM grade, differentiation, and tumor size in osteosarcoma patients; higher circ_ANKIB1 is associated with DXR resistance (DXR-resistant vs DXR-sensitive tissues). |
| Description: |
circ_ANKIB1 is up-regulated in osteosarcoma, especially in DXR-resistant tissues/cells. It promotes DXR chemoresistance and malignant behaviors by sponging miR-26b-5p to upregulate EZH2, which increases resistance-related proteins (MRP1, P-gp, LRP); circ_ANKIB1 knockdown suppresses DXR-resistant tumor growth in vivo. |
| Confidence score: |
0.7631 |