| Expression pattern: |
UP |
| Associated gene: |
SKP2-NM_001243120.2, c-Myc |
| Associated microRNA: |
- |
| Biological function: |
promotes MM progression by enhancing proliferation, increasing G2/M cell-cycle proportion, and promoting clonal expansion; contributes to bone marrow microenvironment interference via exosomal secretion |
| Molecular mechanism: |
circHNRNPU encodes a 603-aa protein isoform that regulates alternative splicing (SKP2 exon skipping), leading to reduced c-Myc ubiquitination and stabilization of c-Myc; circHNRNPU is secreted via exosomes to affect BM niche cells |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ubiquitination (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; RIP (RNA Immunoprecipitation); RNA-seq; Western Blot; IF (Immunofluorescence); Transwell Assay; Co-IP |
| Clinical significance: |
Increased circHNRNPU was associated with poor outcomes in four independent MM patient cohorts; higher circHNRNPU expression was associated with inferior EFS survival; proposed as diagnostic biomarker/therapeutic marker |
| Description: |
circHNRNPU (hsa_circ_0017272) is up-regulated in IgD MM/MM and associated with inferior clinical outcomes. It is secreted via exosomes and can be translated into circHNRNPU_603aa, which promotes MM proliferation and cell-cycle progression by regulating SKP2 exon skipping to reduce c-Myc ubiquitination and stabilize c-Myc. |
| Confidence score: |
0.8792 |