| Expression pattern: |
UP |
| Associated gene: |
IGF1R |
| Associated microRNA: |
miR-6838-5p |
| Biological function: |
promotes proliferation, migration and invasion (metastasis) of osteosarcoma cells; promotes osteosarcoma progression |
| Molecular mechanism: |
ceRNA mechanism: circRASSF2 sponges miR-6838-5p to restore/promote IGF1R expression, leading to PI3K/AKT pathway activation |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Wound Healing Assay; Transwell Assay; Clinical Sample Validation |
| Clinical significance: |
High circRASSF2 levels were associated with higher clinical stage and distant metastasis of osteosarcoma |
| Description: |
circRASSF2 is up-regulated in osteosarcoma cells and tissues and promotes proliferation, migration/invasion and metastasis-related behaviors. Mechanistically, it acts as a ceRNA to sponge miR-6838-5p, restoring IGF1R expression and activating downstream PI3K/AKT signaling. Clinically, higher circRASSF2 levels are associated with advanced stage and distant metastasis. |
| Confidence score: |
0.7164 |