| Expression pattern: |
UP |
| Associated gene: |
TCF7L2, PI3K/AKT/mTOR pathway |
| Associated microRNA: |
miR-509-3p |
| Biological function: |
Promotes breast cancer cell proliferation and angiogenesis, inhibits apoptosis, and aggravates malignant cellular progression. |
| Molecular mechanism: |
Circ-PRMT5 acts as a ceRNA sponge for miR-509-3p, upregulates TCF7L2, and activates the PI3K/AKT/mTOR pathway. |
| Biological pathway or process: |
PI3K/AKT (promotes); PI3K/AKT/mTOR (promotes); proliferation (promotes); angiogenesis (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Tube Formation Assay; Western Blot; FISH / smFISH; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Survival Analysis |
| Clinical significance: |
Elevation of circ-PRMT5 indicates a poor prognosis in patients with breast cancer; high circ-PRMT5 expression was negatively related to overall survival time. |
| Description: |
Circ-PRMT5 is upregulated in breast cancer tissues and cell lines, and high expression is associated with poor overall survival. It promotes malignant phenotypes by sponging miR-509-3p, increasing TCF7L2 expression, and activating the PI3K/AKT/mTOR pathway, thereby enhancing proliferation and angiogenesis while suppressing apoptosis. |
| Confidence score: |
0.7941 |