circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: circHIPK3
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005055
MONDO name: Kaposi's sarcoma
Disease details: Kaposi’s sarcoma
Disease DO ID:
 8632
Disease MeSH ID:
 D012514
Disease NCIt ID:
 C9087
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

TREx-BCBL1-RTA; HEK-293T; BC-3; HEK-293T-rKSHV.219
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: DLL4, Ago2, ORF57
Associated microRNA: miR-30c, miR-29b
Biological function: Enhances KSHV lytic replication and infectious virion production; contributes to cell cycle dysregulation during lytic replication.
Molecular mechanism: Acts as a competing endogenous RNA (miRNA sponge) for miR-30c (and miR-29b), increasing the miR-30c target DLL4; associates with Ago2; circHIPK3 upregulation is enhanced by KSHV ORF57.
Biological pathway or process:

ceRNA regulation (promotes); cell cycle (other)
Detected method:
Q
Validation methods:

divergent primers PCR; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot
Clinical significance:

-
Description:

During KSHV lytic replication in B cells, circHIPK3 is up-regulated and mainly localized in the cytoplasm. It acts as a ceRNA/miRNA sponge for miR-30c (and miR-29b), resulting in increased DLL4 expression and promoting cell-cycle changes that support efficient viral lytic replication and infectious virion production.
Confidence score:

0.6634
Other information
Title:

Dysregulation of the miR-30c/DLL4 axis by circHIPK3 is essential for KSHV lytic replication.

Journal: EMBO reports
Published: 2022
PubMed ID: 35239998
Study type:

biological research
Data availability: GSE186652
Code availability: -