circRNA basic information
circBase ID: hsa_circ_0067934
Name: hsa_circ_PRKCI
Synonym: circPRKCI / circ_0067934
Host Gene: PRKCI
Genomic location(hg19): chr3:170013698-170015181:+
Genomic location(hg38): chr3:170295910-170297393:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer / TNBC
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; adjacent normal tissues; tumor tissue; TNBC tissues
Cell lines:

MDA-MB-231; BT-549; HCC-1937; MCF-10A; HEK-293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: WBP2, AKT, EIF4A3, Ago2
Associated microRNA: miR-545-3p
Biological function: promotes proliferation; promotes migration; promotes tumor growth in vivo
Molecular mechanism: ceRNA (miRNA sponge) mechanism via miR-545-3p to upregulate WBP2 and promote AKT phosphorylation; EIF4A3 promotes circPRKCI biogenesis and nuclear export
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); migration (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

high circPRKCI expression was positively associated with TNM stage, tumor size and lymph node metastasis, and was correlated with poor prognosis of patients with BC
Description:

circPRKCI (hsa_circ_0067934), derived from PRKCI, is up-regulated in TNBC tissues and cell lines and promotes TNBC proliferation, migration, and xenograft tumor growth. Mechanistically, circPRKCI acts as a miR-545-3p sponge to increase WBP2 and enhance AKT phosphorylation, activating PI3K/AKT signaling; EIF4A3 promotes circPRKCI expression and nuclear export.
Confidence score:

0.8532
Other information
Title:

EIF4A3-mediated circPRKCI expression promotes triple-negative breast cancer progression by regulating WBP2 and PI3K/AKT signaling pathway.

Journal: Cell death discovery
Published: 2022
PubMed ID: 35236829
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1038/s41420-022-00892-y; The circRNAs sequence data were obtained from circbase (http://circrna.org/).
Code availability: -