circRNA basic information
circBase ID: -
Name: hsa_circ_AKT3
Synonym: circ_AKT3
Host Gene: AKT3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

cancer tissues
Cell lines:

MKN-7; HGC-27; MKN-7/DDP; HGC-27/DDP
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PTPN14, P-gp
Associated microRNA: miR-206
Biological function: promotes cisplatin resistance; decreases apoptosis; promotes xenograft tumor growth
Molecular mechanism: ceRNA mechanism: circ_AKT3 sponges miR-206 to regulate PTPN14, affecting cisplatin resistance
Biological pathway or process:

apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; Western Blot; Annexin V/PI Flow Cytometry; In Vivo Animal Model
Clinical significance:

-
Description:

circ_AKT3 is up-regulated in cisplatin-resistant gastric cancer tissues and resistant cell lines. Functionally, circ_AKT3 promotes cisplatin resistance (higher IC50 and P-gp, lower apoptosis) and enhances tumor growth in a cell line-derived xenograft model by sponging miR-206 to upregulate PTPN14 (ceRNA axis).
Confidence score:

0.7032
Other information
Title:

circ_AKT3 knockdown suppresses cisplatin resistance in gastric cancer.

Journal: Open medicine (Warsaw, Poland)
Published: 2022
PubMed ID: 35233464
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -