| Expression pattern: |
UP |
| Associated gene: |
TRIM71, Ago2 |
| Associated microRNA: |
miR-637 |
| Biological function: |
promotes HCC cell proliferation, migration, invasion, cell cycle progression, aerobic glycolysis and tumor growth, and inhibits apoptosis |
| Molecular mechanism: |
circ_0007429 acts as a miR-637 sponge to promote TRIM71 expression, regulate Ago2, and enhance aerobic glycolysis and malignant phenotypes in HCC |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); glycolysis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circ_0007429 expression correlated with tumor size and worse 5-year overall survival in HCC patients |
| Description: |
circ_0007429 is an upregulated RREB1-derived cytoplasmic circRNA in HCC. It promotes HCC progression by sponging miR-637 to increase TRIM71 and regulate Ago2, thereby enhancing proliferation, migration, invasion, cell cycle progression, aerobic glycolysis and tumor growth while suppressing apoptosis. High circ_0007429 expression is associated with larger tumor size and worse overall survival. |
| Confidence score: |
0.8673 |