| Expression pattern: |
UP |
| Associated gene: |
METTL3, CKB, c-Jun |
| Associated microRNA: |
- |
| Biological function: |
promotes breast cancer bone metastasis, skeletal lesions, osteolytic damage, cell proliferation, and cell migration |
| Molecular mechanism: |
METTL3-mediated m6A modification enhances circWDR85 expression; circWDR85 recruits CKB to enhance c-Jun T91 phosphorylation and stabilizes c-Jun protein by inhibiting ubiquitin-mediated degradation |
| Biological pathway or process: |
metastasis (promotes); proliferation (promotes); migration (promotes); m6A modification (other); ubiquitination (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; MeRIP / MeRIP-seq; Transfection; CCK8; Transwell Assay; Western Blot; Co-IP; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
Clinical validation in a large BC patient cohort is still lacking |
| Description: |
circWDR85 is an m6A-modified oncogenic circRNA up-regulated in highly metastatic breast cancer cells and bone metastatic tissues. METTL3 enhances circWDR85 biogenesis through m6A modification, and circWDR85 promotes osteolytic bone metastasis by recruiting CKB to phosphorylate and stabilize c-Jun. |
| Confidence score: |
0.7995 |