| Expression pattern: |
UP |
| Associated gene: |
PDE4A |
| Associated microRNA: |
miR-1306-5p |
| Biological function: |
circAXL knockdown alleviates Abeta1-42-induced cell cytotoxicity, apoptosis, inflammation, oxidative stress and endoplasmic reticulum (ER) stress in SK-N-SH cells. |
| Molecular mechanism: |
circAXL acts as a ceRNA to sponge miR-1306-5p and thereby upregulates PDE4A. |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); oxidative stress (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; ELISA; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circAXL had diagnostic value for AD. |
| Description: |
In an Abeta1-42-induced AD cellular model, circAXL is up-regulated and promotes neuronal injury phenotypes (reduced viability, increased apoptosis, inflammation, oxidative and ER stress). Mechanistically, circAXL sponges miR-1306-5p to derepress PDE4A. Serum exosomal circAXL is elevated in AD patients and shows diagnostic potential by ROC analysis. |
| Confidence score: |
0.8314 |