| Expression pattern: |
UP |
| Associated gene: |
SERPINH1 mRNA, SERPINH1, c-Myc, SRSF10 |
| Associated microRNA: |
- |
| Biological function: |
Promotes NPC cell proliferation, migration, invasion, tumor growth and metastasis. |
| Molecular mechanism: |
circCAMSAP1 binds the SERPINH1 mRNA 3’UTR to increase SERPINH1 mRNA stability and expression; SERPINH1 binds c-Myc to inhibit its ubiquitination-degradation, increasing c-Myc; c-Myc (with SRSF10) promotes CAMSAP1 transcription and back-splicing to enhance circCAMSAP1 production (positive feedback loop). |
| Biological pathway or process: |
mRNA stability (promotes); ubiquitination (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; MTT; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; circRIP; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; IF (Immunofluorescence); ChIP / ChIP-seq; Bioinformatics Analysis |
| Clinical significance: |
circCAMSAP1 expression was significantly correlated with high T stage, N stage, and M stage classification in NPC patients; suggested as a prognostic biomarker or therapeutic target. |
| Description: |
circCAMSAP1 is up-regulated in nasopharyngeal carcinoma and promotes proliferation and metastasis. Mechanistically, circCAMSAP1 binds the SERPINH1 mRNA 3’UTR to enhance SERPINH1 mRNA stability and expression; SERPINH1 then stabilizes c-Myc by inhibiting its ubiquitination-degradation, while c-Myc (with SRSF10) further increases circCAMSAP1 biogenesis, forming a positive feedback loop. |
| Confidence score: |
0.8489 |