circRNA basic information
circBase ID: hsa_circ_0041732
Name: hsa_circ_FAM64A
Synonym: circFAM64A
Host Gene: FAM64A
Genomic location(hg19): chr17:6350782-6351078:+
Genomic location(hg38): chr17:6447462-6447758:+
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

bladder cancer tissues; adjacent normal tissues; peripheral blood; xenograft tissues
Cell lines:

BIU87; UMUC3; T24; 5637; 253 J; RT4; SV-HUC-1; HEK293T; J82
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IL-6, JAK2, STAT3, PD-L1, HIF-1alpha, FAM64A
Associated microRNA: miR-149-5p
Biological function: Promotes bladder cancer cell proliferation and invasion, promotes immune evasion, and inhibits CD8+ T cell killing function.
Molecular mechanism: CircFAM64A(3) acts as a miR-149-5p sponge, relieves miR-149-5p-mediated repression of IL-6, activates the IL-6/JAK/STAT pathway, increases PD-L1 expression, and is transcriptionally induced by HIF-1alpha under hypoxia via the FAM64A promoter.
Biological pathway or process:

JAK/STAT (promotes); proliferation (promotes); invasion (promotes); immune regulation (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; ELISA; RNA-seq; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）
Clinical significance:

High circFAM64A(3) expression was associated with poor prognosis, worse OS and PFS, and a tendency toward muscle invasive bladder cancer.
Description:

CircFAM64A(3), also identified as hsa_circ_0041732, is up-regulated in bladder cancer and high expression is associated with poor prognosis. Functionally, it promotes bladder cancer proliferation and invasion and suppresses CD8+ T cell-mediated antitumor killing. Mechanistically, hypoxia-induced HIF-1alpha promotes circFAM64A(3) transcription, and circFAM64A(3) sponges miR-149-5p to increase IL-6, activate the JAK/STAT pathway, and elevate PD-L1 expression.
Confidence score:

0.8942
Other information
Title:

CircFAM64A(3) promoted bladder cancer proliferation and inhibited CD8 + T cell via sponging to miR-149-5p and activated IL-6/JAK/STAT pathway.

Journal: International immunopharmacology
Published: 2025
PubMed ID: 39662265
Study type:

combined biological and clinical study
Data availability: GSE92675; GSE112719; TCGA; Data will be made available on request; Supplementary data to this article can be found online at https://doi. org/10.1016/j.intimp.2024.113762
Code availability: -