circRNA basic information
circBase ID: hsa_circ_0000885
Name: -
Synonym: circ_0000885 / circ0000885
Host Gene: -
Genomic location(hg19): chr19:7267355-7267907:-
Genomic location(hg38): chr19:7267344-7267896:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung tissues
Cell lines:

HOS; 143B; MG63; KH-OS; U2OS; SaoS2; hFOB1.19; HEK293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F3
Associated microRNA: miR-16-5p
Biological function: promotes osteosarcoma cell proliferation, migration/invasion, tumor growth and metastasis; promotes cancer stem cell differentiation/stemness features
Molecular mechanism: sponges miR-16-5p to enhance E2F3 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; Bioinformatics Analysis; Transfection; Transwell Assay; Colony Formation Assay; CCK8; Western Blot; In Vivo Animal Model; H&E Staining
Clinical significance:

Upregulation of hsa_circ_0000885 indicated a poor clinical outcome of osteosarcoma; candidate biomarker for diagnostics of OS
Description:

hsa_circ_0000885 is up-regulated in osteosarcoma cell lines and acts as an oncogenic circRNA. Its knockdown suppresses proliferation, invasion/migration, tumor growth and lung metastasis, and reduces stemness markers. Mechanistically, hsa_circ_0000885 sponges miR-16-5p to enhance E2F3, and the miR-16-5p/E2F3 axis mediates these phenotypes.
Confidence score:

0.5634
Other information
Title:

Downregulation of hsa_circ_0000885 suppressed osteosarcoma metastasis and progression via regulating E2F3 expression and sponging miR-16-5p.

Journal: Regenerative therapy
Published: 2022
PubMed ID: 35785045
Study type:

biological research
Data availability: -
Code availability: -