circRNA basic information
circBase ID: hsa_circ_0060927
Name: hsa_circ_CYP24A1
Synonym: -
Host Gene: CYP24A1
Genomic location(hg19): chr20:52773707-52788209:-
Genomic location(hg38): chr20:54157168-54171670:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma / RCC
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RCC tissue; peritumor normal tissue; renal cancer tissue; paired normal tissue
Cell lines:

786-O; 769-P; Caki-1; A498; HK-2; 786-O-luc
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: CMTM-4, Ago2
Associated microRNA: miR-421
Biological function: Inhibits RCC cell proliferation, invasion, migration and metastasis; promotes apoptosis; suppresses tumor growth in vivo.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-421 to induce CMTM-4 expression (circCYP24A1/miR-421/CMTM-4 axis).
Biological pathway or process:

proliferation (inhibits); invasion (inhibits); migration (inhibits); metastasis (inhibits); apoptosis (promotes); EMT (inhibits); ERK (inhibits); PI3K/AKT/mTOR (inhibits); ceRNA regulation (other)
Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Clinical Sample Validation; Survival Analysis
Clinical significance:

Low circCYP24A1 expression is associated with shorter overall survival; circCYP24A1 is prognosis-related in RCC.
Description:

circCYP24A1 (hsa_circ_0060927), derived from CYP24A1, is down-regulated in RCC tissues and cell lines and its low expression is associated with worse overall survival. Functionally, circCYP24A1 suppresses RCC proliferation, invasion, migration and metastasis and promotes apoptosis. Mechanistically, it acts as a ceRNA sponge for miR-421 to upregulate CMTM4, thereby dampening EMT- and MEK/ERK- and AKT/mTOR-related malignant phenotypes.
Confidence score:

0.8793
Other information
Title:

CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421.

Journal: Cell death & disease
Published: 2022
PubMed ID: 35220395
Study type:

combined biological and clinical study
Data availability: The original data of the study can be found in the article/Supplementary Material
Code availability: -