| Expression pattern: |
DN |
| Associated gene: |
SMAD3 |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
Circ-MTCL1 promotes cell migration and proliferation in the HSCR-related cellular context; its down-regulation inhibits migration and proliferation of SH-SY5Y and 293T cells. |
| Molecular mechanism: |
Circ-MTCL1 acts as a miR-145-5p sponge to regulate SMAD3 expression, forming a Circ-MTCL1/miR-145-5p/SMAD3 ceRNA-like regulatory mechanism affecting cell migration and proliferation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot |
| Clinical significance: |
It may provide a new molecular target for the diagnosis of HSCR. |
| Description: |
Circ-MTCL1 is down-regulated in HSCR diseased segment colon tissues. Functional assays indicate that Circ-MTCL1 supports migration and proliferation of SH-SY5Y and 293T cells, while knockdown suppresses these processes. Mechanistically, Circ-MTCL1 sponges miR-145-5p to regulate SMAD3 expression, suggesting a Circ-MTCL1/miR-145-5p/SMAD3 axis relevant to HSCR development and potential diagnosis. |
| Confidence score: |
0.778 |