| Expression pattern: |
DN |
| Associated gene: |
p53 |
| Associated microRNA: |
miR-100 |
| Biological function: |
Promotes autophagy and enhances temozolomide (TMZ) sensitivity in p53 wild-type glioblastoma; promotes TMZ-induced apoptosis. |
| Molecular mechanism: |
hsa_circ_0072309 regulates p53 signaling in wild-type p53 GBM by miR-100 mediation and inhibition of p53 ubiquitination, stabilizing p53 protein; p53 signaling is required for circRNA-mediated autophagy, which contributes to TMZ sensitivity. |
| Biological pathway or process: |
autophagy (promotes); p53 signaling (promotes); apoptosis (promotes); chemoresistance (inhibits); ceRNA regulation (other); ubiquitination (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; IF (Immunofluorescence); Annexin V/PI Flow Cytometry; Western Blot; RIP (RNA Immunoprecipitation); Co-IP; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low hsa_circ_0072309 expression predicts poor prognosis for glioma patients; glioma patients with low hsa_circ_0072309 expression had worse survival than those with high hsa_circ_0072309 expression. |
| Description: |
In glioblastoma, hsa_circ_0072309 is downregulated and higher expression is associated with better patient survival. Functionally, it promotes autophagy and increases TMZ sensitivity in p53 wild-type GBM by stabilizing p53 (reducing p53 ubiquitination) via miR-100 mediation, while showing little effect in p53-mutant GBM. |
| Confidence score: |
0.7537 |